用微信扫描上方二维码添加

或打开微信搜索编辑老师微信号添加:

期刊汇官方网站,咨询热线:400-803-1233

《实用医学杂志》入编《中文核心期刊要目总览》2017年版(第8版)之综合性医药卫生类核心期刊

摘要: <正>近日,《实用医学杂志》收到北京大学图书馆《中文核心期刊要目总览》2017年版(第8版)入编通知,这是本刊继2008、2011、2014年之后又一次入选《中文核心期刊要目总览》之综合性医药卫生类核心期刊。《中文核心期刊要目总览》由北京大学图书馆出版,在我国期刊学术界具有较高权威性,是学术期刊优质论文的衡量标尺。2017年版《中文核心期刊要目总览》对核心期刊的评价是采用定量评价和定性评审相结合的方法。其中,定量评价指标体系采用了被摘量(全文、摘要)、被摘率(全文、摘要)、被引量、他引量(期刊、博士论文、会议)、影响因子、他引影

  • 长链非编码RNA在食管癌中的研究进展

    作者:李国良;朱明闯;熊鹏;朱珉; 期刊:《实用医学杂志》 2018年24期

    国家自然科学基金(编号:81172786); ;同济医院基金(编号:2016hgryzm); ;食管癌是一种男性占主导地位的侵袭性恶性肿瘤,死亡率高。世界卫生组织最新数据显示全世界每年约45万余人患病,我国更是食管癌的高发地区,每年新发病例约20万余人,位居世界第一。目前,尽管诊断和治疗技术有了很大进步,但食管癌的治疗效果仍不令人满意。在过去的几十年中,长链非编码RNA(lncRNA)被认为是"转录噪声"或"假基因",因此被忽略。但近年来,越来越多的研究表明,许多lncRNAs与肿瘤发生相关,如果能找到食管癌早期诊断和预后评估相关的lncRNAs,那么这将对我国食管癌的防治工作产生巨大影响。本文主要总结lncRNAs在食管癌中的功能,重点综述由lncRNA介导的影响食管癌生物学特性的调控机制。
    关键词:食管癌;长链非编码RNA;肿瘤标志物;
    基金:国家自然科学基金(编号:81172786); ;同济医院基金(编号:2016hgryzm); ;

    参考文献:
    [1]Cancer statistics in China, 2015[J] . Wanqing Chen,Rongshou Zheng,Peter D. Baade,Siwei Zhang,Hongmei Zeng,Freddie Bray,Ahmedin Jemal,Xue Qin Yu,Jie He. CA: A Cancer Journal for Clinicians . 2016 (2)
    [2]Long non‐coding RNA 91H contributes to the occurrence and progression of esophageal squamous cell carcinoma by inhibiting IGF2 expression[J] . Tianyi Gao,Bangshun He,Yuqin Pan,Yeqiong Xu,Rui Li,Qiwen Deng,Huilin Sun,Shukui Wang. Mol. Carcinog. . 2015 (5)
    [3]Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012[J] . Jacques Ferlay,Isabelle Soerjomataram,Rajesh Dikshit,Sultan Eser,Colin Mathers,Marise Rebelo,Donald Maxwell Parkin,David Forman,Freddie Bray. Int. J. Cancer . 2015 (5)
    [4]Long Noncoding RNAs: Emerging Stars in Gene Regulation, Epigenetics and Human Disease[J] . Arunoday Bhan,Subhrangsu S. Mandal. ChemMedChem . 2014 (9)
    [5]Increased Levels of the Long Intergenic Non-protein Coding RNA POU3F3 Promote DNA Methylation in Esophageal Squamous Cell Carcinoma Cells[J] . Wei Li,Jian Zheng,Jieqiong Deng,Yonghe You,Hongchun Wu,Na Li,Jiachun Lu,Yifeng Zhou. Gastroenterology . 2014
    [6]Upregulation of the long non‐coding rna hotair promotes esophageal squamous cell carcinoma metastasis and poor prognosis[J] . Fang‐Jun Chen,Ming Sun,Su‐Qing Li,Qing‐Quan Wu,Lv Ji,Zhi‐Li Liu,Guo‐Zhi Zhou,Gang Cao,Lei Jin,Hai‐Wei Xie,Chun‐Mei Wang,Jin Lv,Wei De,Ming Wu,Xiu‐Feng Cao. Mol. Carcinog. . 2013 (11)
    [7]Long non-coding RNAs: A new frontier in the study of human diseases[J] . Xuefei Shi,Ming Sun,Hongbing Liu,Yanwen Yao,Yong Song. Cancer Letters . 2013 (2)
    [8]Hypomethylation of Noncoding DNA Regions and Overexpression of the Long Noncoding RNA, AFAP1-AS1 , in Barrett’s Esophagus and Esophageal Adenocarcinoma[J] . Wenjing Wu,Tushar D. Bhagat,Xue Yang,Jee Hoon Song,Yulan Cheng,Rachana Agarwal,John M. Abraham,Sariat Ibrahim,Matthias Bartenstein,Zulfiqar Hussain,Masako Suzuki,Yiting Yu,Wei Chen,Charis Eng,John Greally,Amit Verma,Stephen J. Meltzer. Gastroenterology . 2013 (5)
    [1] Long non-coding RNA GAS5promotes proliferation,migration and invasion by regulation of miR-301ain esophageal cancer. Li W,Zhao W,Lu Z,et al. Oncology Research . 2018
    [2] The bright side of dark matter,lnc RNAs in cancer. Evans J R,Feng F Y,Chinnaiyan A M. The Journal of Clinical Investigation . 2016
    [3] Overview of esophageal cancer. Abbas G,Krasna M. Ann Cardiothorac Surg . 2017
    [4] Long Noncoding RNAs in Cancer Pathways. Schmitt AM,Chang HY. Cancer Cell . 2016
    [5] Long noncoding rna and cancer:a new paradigm. Bhan A,Soleimani M,Mandal SS. Cancer Research . 2017
    [6] Overexpression of long non-coding RNA UCA1 predicts a poor prognosis in patients with esophageal squamous cell carcinoma. Li J Y,Ma X,Zhang C B. International journal of clinical and experimental pathology . 2014
    [7] ce RNA in cancer:possible functions and clinical implications. Qi X L,Zhang D H,Wu N,et al. Journal of Medicine . 2015
    [8] ANRIL inhibits p15 INK4b,through the TGFβ1 signaling pathway in human esophageal squamous cell carcinoma. Chen D,Zhang Z,Mao C,et al. Cellular Immunology . 2014
    [9] Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression. Khalil Ahmad M,Guttman Mitchell,Huarte Maite,Garber Manuel,Raj Arjun,Rivea Morales Dianali,Thomas Kelly,Presser Aviva,Bernstein Bradley E,van Oudenaarden Alexander,Regev Aviv,Lander Eric S,Rinn John L. Proceedings of the National Academy of Sciences of the United States of America . 2009
    [10] Linc RNA-uc002yug.2 involves in alternative splicing of RUNX1 and serves as a predictorfor esophageal cancer and prognosis. Wu H,Zheng J,Deng J,et al. Oncegene . 2015

    食管癌长链非编码RNA肿瘤标志物

  • 经颅聚焦超声刺激的临床研究与应用现状

    作者:李莹萱;林华; 期刊:《实用医学杂志》 2018年24期

    无创神经调控技术具有无痛、安全、操作可重复等优点,其中经颅聚焦超声刺激以及磁共振引导的聚焦超声刺激方兴未艾;基于热消融和非热学效应,其在难治性特发性震颤的治疗方面已取得了一定的成果,未来的临床应用价值不容小觑。本文对其近期临床研究及应用情况加以综述。
    关键词:经颅聚焦超声刺激;磁共振引导的聚焦超声刺激;特发性震颤;神经调控技术;

    参考文献:
    [1]原发性震颤的研究及治疗进展[J]. 乔梁,李勇杰. 立体定向和功能性神经外科杂志. 2017(02)
    [2]靶向超声微泡介导miRNA在疾病治疗中的研究[J]. 郑智唯,赵云,刘朝奇. 实用医学杂志. 2017(04)
    [3]超声神经调控的研究进展[J]. 沈雪莲,严飞,赵云,周军. 临床超声医学杂志. 2016(11)
    [4]经颅超声刺激对帕金森小鼠运动功能及抗氧化能力的影响[J]. 王勇,任佰绪,吴书峰,钟琴,李小俚,路承彪. 中华物理医学与康复杂志. 2015 (07)
    [1]用于经颅神经刺激的超声环型相控阵探头的设计和研制[D]. 黄林冰.深圳大学 2017
    [2]低强度经颅超声对大鼠脑神经刺激作用研究[D]. 马志涛.燕山大学 2017
    [1]MR-guided focused ultrasound thalamotomy for essential tremor: a proof-of-concept study[J] . Nir Lipsman,Michael L Schwartz,Yuexi Huang,Liesly Lee,Tejas Sankar,Martin Chapman,Kullervo Hynynen,Andres M Lozano. Lancet Neurology . 2013 (5)
    [1] Transcranial MRI-guided focused ultrasound:a review of the technologic and neurologic applications. Ghanouni P,Pauly KB,Elias WJ,et al. American Journal of Roentgenology . 2015
    [2] Transcranial focused ultrasound modulates the activity of primary somatosensory cortex in humans. Legon W,Sato T F,Opitz A,et al. Nature Neuroscience . 2014
    [3] Early ultrasonic effects upon mammalian CNS structures (chemical synapses). Borrelli M J,Bailey K I,Dunn F. The Journal of The Acoustical Society of America . 1981
    [4] Ultrasound focusing using magnetic resonance acoustic radiation force imaging: application to ultrasound transcranial therapy. Hertzberg Y,Volovick A,Zur Y,Medan Y,Vitek S,Navon G. Medical Physics . 2010
    [5] Ultrasound for the brain. Landhuis E. Nature . 2017
    [6] Neuromodulation with transcranial focused ultra-sound. Kubanek J. Neurosurgical focus . 2018
    [7] A review of the current therapies,challenges,and future directions of transcranial focused ultrasound technology:Advances in diagnosis and treatment. Krishna V,Sammartino F,Rezai A. JAMA Neurol . 2018
    [8] Focused ultrasound as a non-invasive intervention for neurological disease:a review. Piper RJ,Hughes MA,Moran CM,et al. British Journal of Neurosurgery . 2016
    [9] Unilateral magnetic resonance guided focused ultrasound thalamotomy for essential tremor:practices and clinicoradiological outcomes. Chang WS,Jung HH,Kweon EJ,et al. Journal of Neurology Neurosurgery and Psychiatry . 2015
    [10] A pilot study of focused ultrasound thalamotomy for essential tremor. Elias WJ,Huss D,Voss T,et al. The New England Journal of Medicine . 2013

    经颅聚焦超声刺激磁共振引导的聚焦超声刺激特发性震颤神经调控技术

  • 高糖诱导H9c2心肌细胞TRAP1的表达变化及意义

    作者:钟祯;李万根;张霄旦; 期刊:《实用医学杂志》 2018年24期

    广东省自然科学基金项目(编号:2017A030310257); ;广州市科技计划项目(编号:201707010045); ;广州医科大学博士、留学回国人员科研项目(编号:2015C10); ;目的构建体外高糖诱导的H9c2心肌细胞损伤模型,观察高糖环境下心肌细胞损伤情况及TRAP1的表达变化及意义。方法将体外培养的H9c2细胞随机分为正常糖对照组(5.5 mmol/L葡萄糖)、高糖组(33.0 mmol/L葡萄糖)、高渗组(5.5 mmol/L葡萄糖+27.5 mmol/L甘露醇),体外培养48 h;Western blot及实时荧光定量PCR(qRT-PCR)方法检测各组细胞TRAP1蛋白及mRNA相对表达量;MTS检测心肌细胞活性;2’,7’-二氢二氯荧光黄双乙酸盐(DCFH-DA)荧光探针检测细胞内ROS含量;JC-1荧光染色检测心肌细胞线粒体膜电位变化。结果与正常糖对照组及高渗组相比,高糖组H9c2细胞中TRAP1 mRNA及蛋白表达量均下降(P <0.05);细胞活性下降(P <0.05);细胞内ROS含量增多(P <0.05);心肌细胞线粒体膜电位下降(P <0.05)。结论高糖环境下心肌细胞中TRAP1表达的下降,可能与ROS介导的线粒体功能受损所致的心肌损伤密切相关。
    关键词:糖尿病心肌病;肿瘤坏死因子受体相关蛋白1;线粒体;氧化应激;
    基金:广东省自然科学基金项目(编号:2017A030310257); ;广州市科技计划项目(编号:201707010045); ;广州医科大学博士、留学回国人员科研项目(编号:2015C10); ;

    参考文献:
    [1]糖尿病心肌病患者内源性抗氧化体系及氧化应激水平[J]. 齐晓丹,常凌峰,于海涛,刘颖,高勇,林树东,闫晓光,许冬霞,张春晶. 实用医学杂志. 2018(08)
    [2]糖尿病心肌病发病机制的研究进展[J]. 潘利亚,张晓卉,尹新华. 中国心血管杂志. 2017(02)
    [1]Expression of TRAP1 Predicts Poor Survival of Malignant Glioma Patients[J] . Shuai Li,Qingjie Lv,Hanxue Sun,Yixue Xue,Ping Wang,Libo Liu,Zhiqing Li,Zhen Li,Xin Tian,Yun-Hui Liu. Journal of Molecular Neuroscience . 2015 (1)
    [2]Diabetic cardiomyopathy and its mechanisms: Role of oxidative stress and damage[J] . Quan Liu,Shudong Wang,Lu Cai. J Diabetes Invest . 2014 (6)
    [3]Diabetic cardiomyopathy: Mechanisms and new treatment strategies targeting antioxidant signaling pathways[J] . Karina Huynh,Bianca C. Bernardo,Julie R. McMullen,Rebecca H. Ritchie. Pharmacology and Therapeutics . 2014
    [4]Suppression of tumor necrosis factor receptor‐associated protein 1 expression induces inhibition of cell proliferation and tumor growth in human esophageal cancer cells[J] . Xin Tian,Ping Ma,Cheng‐Guang Sui,Fan‐Dong Meng,Yan Li,Li‐Ye Fu,Tao Jiang,Yang Wang,You‐Hong Jiang. FEBS J . 2014 (12)
    [5]Mitochondrial chaperone tumour necrosis factor receptor‐associated protein 1 protects cardiomyocytes from hypoxic injury by regulating mitochondrial permeability transition pore opening[J] . FeiXiang,Yue‐ShengHuang,Xiao‐HuaShi,QiongZhang. FEBS Journal . 2010 (8)
    [1] Biochemistry and molecular cell biology of diabetic complications. Michael B. Nature . 2001
    [2] Identification of a protein with homology to hsp90 that binds the type 1 tumor necrosis factor receptor. Song H Y,Dunbar J D,Zhang Y X,Guo D,Donner D B. Journal of Biological Chemistry . 1995
    [3] Iron chelation study in a normal human hepatocyte cell line suggests that tumor necrosis factor receptor-associated protein 1 (TRAP1) regulates production of reactive oxygen species. Im Chang-Nim,Lee Jae-Seon,Zheng Ying,Seo Jeong-Sun. Journal of cellular biochemistry . 2006
    [4] New type of cardiomyopathy associated with diabetic glomerulosclerosis. Rubler S,Dlugash J,Yuceoglu Y Z,Kumral T,Branwood A W,Grishman A. The American Journal of Cardiology . 1972
    [5] Diabetic cardiomyopathy:where we are and where we are going. Lee WS,Kim J. Korean Journal of Internal Medicine . 2017
    [6] HSP75 protects against cardiac hypertrophy and fibrosis. Zhang Yan,Jiang Ding-Sheng,Yan Ling,Cheng Kuo-Ju,Bian Zhou-Yan,Lin Guo-Sheng. Journal of cellular biochemistry . 2011
    [7] Overexpression of mitochondrial Hsp70/Hsp75 protects astrocytes against ischemic injury in vitro. LA Voloboueva,M Duan,Y Ouyang,JF Emery,C Stoy,RG Giffard. Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism . 2008
    [8] Association between diabetes and cause-specific mortality in rual and urban areas of China. Bragg F,Holmes M V,Iona A,et al. The Journal of The American Medical Association . 2017
    [9] The Chaperone TRAP1 As a modulator of the mitochondrial adaptations in cancer cells. MASGRAS I,SANCHEZ-MARTIN C,COLOMBO G,et al. Front Oncol . 2017
    [10] TRAP1:a viable therapeutic target for future cancer treatments?. LETTINI G,MADDALENA F,SISINNI L,et al. Expert Opinion on Therapeutic Patents . 2017

    糖尿病心肌病肿瘤坏死因子受体相关蛋白1线粒体氧化应激

  • HBV感染人外周血单个核细胞时体外免疫效应的变化

    作者:梅清;李小鹏;吴振平;李丹;张文苑;余婷婷;张伦理; 期刊:《实用医学杂志》 2018年24期

    江西省自然科学基金项目(编号:20161BAB205254,20161BAB205250); ;南昌大学研究生创新专项基金项目(编号:CX2017239); ;目的观察免疫耐受期的乙肝病毒感染者血清刺激正常人外周血单个核细胞(PBMCs)后出现的免疫效应变化。方法用乙肝病毒的脱氧核糖核酸(HBV DNA)为2×107IU/mL的免疫耐受期的乙肝病毒感染者血清刺激健康人的PBMCs,并设为实验组,刺激的时间一共为24 d,另外设立未用感染者血清刺激的健康人PBMCs为对照组。CCK8法检测实验组和对照组中PBMCs的细胞数,ELISA法检测2组中上清液里干扰素-γ(IFN-γ)和肿瘤坏死因子-α(TNF-α)的浓度,流式细胞术检测CD8~+T细胞上程序性死亡受体-1(PD-1)阳性率。结果在刺激的第8天,实验组中PBMC数量高于对照组,实验组TNF-α浓度[(1 646.89±149.92)pg/mL]高于对照组[(1 061.20±151.29)pg/mL,t=4.76,P=0.009],实验组IFN-γ的浓度[(134.00±15.10)pg/mL]高于对照组[(24.26±8.22)pg/mL,t=11.06,P=0.000]。刺激的第24天时实验组TNF-α浓度[(395.67±25.17)pg/mL]低于对照组[(576.33±15.18)pg/mL,t=-10.65,P=0.000],实验组IFN-γ浓度[(14.05±1.65)pg/mL]低于对照组[(20.75±2.47)pg/mL,t=-3.91,P=0.017]。未开始刺激时,实验组与对照组CD8+T细胞上PD-1阳性率均为(12.23±1.72)%,持续刺激24 d后,实验组与对照组的PD-1表达水平分别为(16.70±1.65)%、(13.17±2.31)%。实验组刺激24 d后PD-1表达水平高于未开始刺激时,且差异有统计学意义(P=0.032),对照组第24天PD-1表达水平相比于未开始刺激时则差异无统计学意义(P=0.605)。结论乙肝病毒刺激PBMCs的初期,细胞数会增殖,并且产生炎症因子IFN-γ和TNF-α;但长期刺激后其炎症因子产生能力减弱,且CD8+T细胞表面抑制性受体PD-1表达上调。
    关键词:乙型肝炎病毒;免疫;外周血单个核细胞;程序性死亡受体-1;干扰素-γ;肿瘤坏死因子-α;
    基金:江西省自然科学基金项目(编号:20161BAB205254,20161BAB205250); ;南昌大学研究生创新专项基金项目(编号:CX2017239); ;

    参考文献:
    [1]乙肝病毒感染慢性化与相关免疫细胞[J]. 杨柳,宋红丽. 实用医学杂志. 2016(06)
    [2]外周血单个核细胞介导乙型肝炎病毒感染的transwell小室体外模型研究[J]. 魏俊妮,张玥,高雪芬,薛淑莲,王素萍. 中华传染病杂志. 2015 (06)
    [3]外周血单个核细胞介导的HBV宫内垂直传播[J]. 周娜,王健. 中国免疫学杂志. 2014(07)
    [4]HBV感染外周血单个核细胞的研究进展[J]. 周永,史昌河,宣世英,董全江,周蓉蓉,孙蓬蓬,魏阳,刘金亭. 中华实验和临床感染病杂志(电子版). 2013(05)
    [5]T cell immunopathogenesis and immunotherapeutic strategies for chronic hepatitis B virus infection[J]. Yukihiro Shimizu. World Journal of Gastroenterology. 2012(20)
    [1]T cell exhaustion during persistent viral infections[J] . Shannon M. Kahan,E. John Wherry,Allan J. Zajac. Virology . 2015
    [2]HBV and the immune response[J] . Carlo Ferrari. Liver Int . 2015
    [3]Restoration of HBV-specific CD8+ T cell function by PD-1 blockade in inactive carrier patients is linked to T cell differentiation[J] . Bertram Bengsch,Bianca Martin,Robert Thimme. Journal of Hepatology . 2014
    [4]Transcriptomic analysis of the woodchuck model of chronic hepatitis B[J] . Simon P. Fletcher,Daniel J. Chin,Yongmei Ji,A. Leonardo Iniguez,Bruce Taillon,David C. Swinney,Palanikumar Ravindran,Donavan T. Cheng,Hans Bitter,Uri Lopatin,Han Ma,Klaus Klumpp,Stephan Menne. Hepatology . 2012 (3)
    [1] Adaptive immunity in HBV infection. Bertoletti A,Ferrari C. Journal of Hepatology . 2016
    [2] Regulation of inflammatory gene expression in PBMCs by immunostimulatory botanicals. Denzler Karen L,Waters Robert,Jacobs Bertram L,Rochon Yvan,Langland Jeffrey O. PloS one . 2010
    [3] Immunopathogenesis of Hepatitis B Virus. Tseng TC,Huang LR. Journal of Infection . 2017
    [4] Research progress of therapeutic vaccines for treating chronic hepatitis B. Li J,Bao M,Ge J,Ren S,Zhou T,Qi F,Pu X,Dou J. Hum Vaccin Immunother . 2017
    [5] Molecular and cellular insights into T cell exhaustion. WHERRY E J,KURACHI M. Nature Reviews Immunology . 2015
    [6] Interferon-γand Tumor Necrosis Factor-αProduced by T Cells Reduce the HBV Persistence Form,ccc DNA,Without Cytolysis. Xia Y,Stadler D,Lucifora J,Reisinger F,Webb D,H?sel M,Michler T,Wisskirchen K,Cheng X,Zhang K,Chou WM,Wettengel JM,Malo A,Bohne F,Hoffmann D,Eyer F,Thimme R,Falk CS,Thasler WE,Heikenwalder M,Protzer U. Gastroenterology . 2016
    [7] Control of hepatitis B virus by cytokines. Xia YC,Protzer U. Viruses . 2017
    [8] T-cell exhaustion in chronic hepatitis B infection:current knowledge and clinical significance. Ye B,Liu X,Li X,Kong H,Tian L,Chen Y. Cell Death Dis . 2015
    [9] Programmed Cell Death 1 (PD-1)and Cytotoxic T Lymphocyte-Associated Antigen 4 (CTLA-4)in Viral Hepatitis. Hyosun Cho,Hyojeung Kang,Hwan Hee Lee.et al. International Journal of Molecular Sciences . 2017
    [10] Demethylation of the PD-1promoter is imprinted during the effector phase of CD8 T cell exhaustion. Ahn E,Youngblood B,Lee J,et al. Journal of Virology . 2016

    乙型肝炎病毒免疫外周血单个核细胞程序性死亡受体1干扰素γ肿瘤坏死因子α

  • 贲门胃底癌中CIP2A mRNA表达与HER-2基因的扩增及其意义

    作者:张洪兰;陈昊;张春芳;张昶;齐冬雪;李伟;刘新丽;刘华; 期刊:《实用医学杂志》 2018年24期

    连云港市科技局社会发展计划学科建设课题资助(编号:SH1325); ;目的检测贲门胃底癌中CIP2A mRNA表达与HER-2基因的扩增并探讨其相关性和临床意义。方法采用半定量RT-PCR法检测47例贲门胃底癌石蜡包埋组织中CIP2A mRNA的转录水平,荧光原位杂交方法(FISH)检测HER-2基因的扩增情况,统计分析CIP2A mRNA与HER-2扩增情况的相关性,同时了解二者与贲门胃底癌患者临床病理参数的关系。另选取47例对应的癌旁组织纳入半定量RT-PCR方法检测,并统计分析CIP2A mRNA在癌和癌旁组织中转录水平的差异。结果 CIP2A mRNA高水平转录与肠型胃癌、较高的分化程度、较大的肿瘤浸润深度、较高的TNM分期、血管癌栓、神经侵犯等临床病理参数相关(P <0.05);HER-2扩增与肠型胃癌、较高的分化程度、神经侵犯等临床病理参数相关(P <0.05)。CIP2AmRNA高水平转录与HER-2扩增呈正相关。贲门胃底癌组织中CIP2A m RNA的转录水平显著高于其对应的癌旁组织(P <0.05)。结论 CIP2A基因在贲门胃底癌组织中高表达,与患者的恶性临床病理参数密切相关。CIP2A基因可能成为贲门胃底癌患者新的分子标志物和治疗靶点,其可受到HER-2正调节。
    关键词:贲门胃底癌;CIP2A;HER-2;石蜡包埋组织;预后;RT-PCR;FISH;
    基金:连云港市科技局社会发展计划学科建设课题资助(编号:SH1325); ;

    参考文献:
    [1]潮汕地区902例胃癌原发病灶中表皮生长因子受体2表达及其临床意义[J]. 况丽平,詹晓芬,赵勇强,刘春鹏,王少洪. 实用医学杂志. 2017(20)
    [2]曲妥珠单抗联合长春瑞滨治疗HER-2阳性晚期乳腺癌患者的疗效分析[J]. 崔剑锋. 实用药物与临床. 2017(07)
    [3]CIP2A表达与贲门胃底癌微血管密度、进展及预后的关系[J]. 张洪兰,陈昊,张春芳,张昶,齐冬雪. 广东医学. 2017(07)
    [4]贲门胃底癌神经旁浸润与CIP2A表达及其预后意义[J]. 张洪兰,陈昊,张春芳,张昶,齐冬雪,刘华. 临床与实验病理学杂志. 2016(08)
    [5]RhoA和C-myc在胃癌发生发展中的表达及意义[J]. 王莉,张旭,刘丽秋,孙玉鸿,朱艳丽,赵玉德,江清林. 实用医学杂志. 2014(19)
    [6]胃癌TNM分期标准第6版和第7版对胃癌术后预后判断的价值比较[J]. 洪骏,沈玉根,毕建威. 第二军医大学学报. 2012(05)
    [7]CIP2A在肝癌组织中的表达及其临床意义[J]. 杨雪,宋涛,杨威,郭成,姚英民,刘青光. 西安交通大学学报(医学版). 2011(04)
    [8]胃癌中HER-2/neu基因扩增和蛋白表达的多中心研究[J]. HER-2研究全国协作组,张瑰红. 中华消化杂志. 2006(10)
    [9]行根治术治疗食管癌和贲门癌患者的预后分析[J]. 刘巍,郝希山,范倩,李海欣,宋丽楠,王士杰,王培忠,晋颖,陈勇,关丽云,平育敏,孟宪利,王瑞,刘俊锋,王小玲. 中华肿瘤杂志. 2008 (12)
    [1]PolyA PCR Amplification of cDNA from RNA Extracted from Formalin-Fixed Paraffin-Embedded Tissue[J] . Richard Byers,Jamie Roebuck,Ebrahim Sakhinia,Judith Hoyland. Diagnostic Molecular Pathology . 2004 (3)
    [2]RT-PCR Analysis of RNA Extracted from Bouin-Fixed and Paraffin-Embedded Lymphoid Tissues[J] . Annunziata Gloghini,Barbara Canal,Ulf Klein,Luigino Dal Maso,Tiziana Perin,Riccardo Dalla-Favera,Antonino Carbone. The Journal of Molecular Diagnostics . 2004 (4)
    [3]HER2 signaling enhances 5′UTR‐mediated translation of c‐Myc mRNA[J] . EnricoGalmozzi,PatriziaCasalini,Marilena ValeriaIorio,BarbaraCasati,CleliaOlgiati,SylvieMénard. J. Cell. Physiol. . 2004 (1)

    贲门胃底癌CIP2AHER2石蜡包埋组织预后RTPCRFISH

  • HOXB7在特发性肺纤维化中抗纤维化的作用

    作者:周淼;李风雷;张海龙;孙俊波; 期刊:《实用医学杂志》 2018年24期

    国家中医临床研究基地业务建设科研专项(编号:JDZX2015158); ;河南中医学院博士科研基金(编号:BSJJ2015-27); ;目的探讨抑制HOXB7对TGF-β1诱导的肺泡上皮细胞纤维化的影响及可能的相关机制。方法 qRT-PCR检测肺纤维化组织中HOXB7的mRNA表达量;TGF-β1诱导A549纤维化;转染HOXB7siRNA到A549细胞,抑制HOXB7;倒置相差显微镜观察细胞形态变化;qRT-PCR检测A549细胞HOXB7、E-cadherin、α-SMA和COL1A1的mRNA表达量;Western blot检测A549细胞HOXB7、E-cadherin、α-SMA、bFGF和COL1A1蛋白表达量。细胞分组:control组(空白对照)、TGF-β1组(TGF-β1诱导)、β1-HOXB7 si组(TGF-β1诱导后转染HOXB7 siRNA)、β1-non-spe组(TGF-β1诱导后转染non-specific siRNA)、β1-HOXB7si-bFGF组(bFGF处理的β1-HOXB7 si组)。结果肺纤维组织HOXB7的mRNA表达量显著升高;细胞转染实验中HOXB7的mRNA和蛋白表达量均显著降低;降低HOXB7表达量显著抑制A549向细胞纤维化形态改变;抑制HOXB7显著升高E-cadherin mRNA和蛋白表达量,且降低α-SMA和COL1A1的mRNA和蛋白表达量;抑制HOXB7显著降低bFGF的蛋白表达量,且bFGF孵育细胞可有效反转抑制HOXB7对细胞E-cadherin、α-SMA和COL1A1表达量的影响。结论抑制HOXB7可通过调控bFGF有效降低TGF-β1诱导的肺泡细胞EMT过程,进而抑制其纤维化。
    关键词:HOXB7;Egr-1;EMT;Ⅱ型肺泡上皮细胞;IPF;
    基金:国家中医临床研究基地业务建设科研专项(编号:JDZX2015158); ;河南中医学院博士科研基金(编号:BSJJ2015-27); ;

    参考文献:
    [1]USP22对宫颈癌细胞增殖及顺铂化疗敏感性的影响[J]. 刘春艳,毛熙光. 实用医学杂志. 2018(02)
    [1]Distinct mechanisms for opposite functions of homeoproteins Cdx2 and HoxB7 in double-strand break DNA repair in colon cancer cells[J] . Christine Soret,Elisabeth Martin,Isabelle Duluc,Fran?oise Dantzer,Marie Vanier,Isabelle Gross,Jean-No?l Freund,Claire Domon-Dell. Cancer Letters . 2016
    [2]BCL6 induces EMT by promoting the ZEB1-mediated transcription repression of E-cadherin in breast cancer cells[J] . Jin-Mei Yu,Wei Sun,Fang Hua,Jing Xie,Heng Lin,Dan-Dan Zhou,Zhuo-Wei Hu. Cancer Letters . 2015 (2)
    [1] Mi R-210promotes IPF fibroblast proliferation in response to hypoxia. BODEMPUDI V,HERGERT P,SMITH K,et al. American Journal of Physiology Lung Cellular and Molecular Physiology . 2014
    [2] ADAM10-mediated ephrin-B2 shedding promotes myofibroblast activation and organ fibrosis. Lagares D,Ghassemikakroodi P,Tremblay C,et al. Nature Medicine . 2017
    [3] Estrogens do not protect,but androgens exacerbate,collagen accumulation in the female mouse kidney after ureteric obstruction. Hewitson TD,Boon WC,Simpson ER,et al. Life Sciences . 2016
    [4] DA-Raf-Mediated Suppression of the Ras-ERK Pathway Is Essential for TGF-betal-Induced Epithelial-Mesenchymal Transition in Alveolar Epithelial Type 2 Cells. Watanabe-Takano, H,Takano, K,Hatano, M,Tokuhisa, T,Endo, T. PloS one . 2015
    [5] Size-and shape-dependent foreign body immune response to materials implanted in rodents and non-human primates. Veiseh 0,Doloff JC,Ma M,et al. Nanostructured Materials . 2015
    [6] The Widening Sphere of Influence of HOXB7 in Solid Tumors. Errico MC,Jin K,Sukumar S.et al. Cancer Research . 2016
    [7] HOXB7 accelerates the malignant progression of hepatocellular carcinoma by promoting stemness and epithelial-mesenchymal transition. HUAN H B,YANG D P,WEN X D,et al. Journal of Experimental and Clinical Cancer Research . 2017
    [8] Fibroblast growth factor-1attenuates TGF-β1-induced lung fibrosis. Shimbori C,Bellaye P S,Xia J,et al. Journal of Paleopathology . 2016
    [9] Signaling pathways and their miRNA regulators involved in the etiopathology of idiopathic pulmonary fibrosis (IPF)and hypersensitivity pneumonitis (HP). Kiszalkiewicz J,Piotrowski W,Brzezianska-Lasota E. Adv Respir Med . 2017
    [10] NOGO-B promotes EMT in lung fibrosis via MMP14 mediates free TGF-betal formation. Xiong Y,Zhang J,Shi L et al. Oncotarget . 2017

    HOXB7EGR1Ⅱ型肺泡上皮细胞

  • 高尿酸对肾小管上皮细胞HK-2增殖、凋亡的影响及其机制研究

    作者:曾宇鹏;谢席胜; 期刊:《实用医学杂志》 2018年24期

    目的探究高尿酸对肾小管上皮细胞增殖、凋亡的影响及其分子机制的研究。方法使用0、50、100、150、200、250、300 mg/L的尿酸处理肾小管上皮细胞24 h,以及150 mg/L的尿酸处理肾小管上皮细胞24、48、72 h,采用四甲基偶氮唑蓝(MTT)方法检测肾小管上皮细胞增殖能力的变化;流式细胞仪检测尿酸对肾小管上皮细胞凋亡的影响;应用蛋白免疫印迹法(Western blot)检测尿酸处理后肾小管上皮细胞Caspase-3/Cleaved-Caspase 3、Caspase-12/Cleaved-Caspase-12、BiP/GRP78、IRE1和JNK蛋白表达水平变化。结果尿酸抑制肾小管上皮细胞增殖能力,并呈浓度依赖性和时间依赖性;不同浓度尿酸处理后,肾小管上皮细胞Cleaved-Caspase 3和Cleaved-Caspase 12蛋白表达上调,流式细胞仪检测细胞凋亡率升高;尿酸激活肾小管上皮细胞内质网应激,BiP/GRP78、IRE1和JNK蛋白表达上调;与尿酸组相比,尿酸+4-PBA处理后细胞凋亡有所改善。结论尿酸通过激活内质网应激,抑制肾小管上皮细胞增殖并促进其凋亡。
    关键词:肾小管上皮细胞;尿酸;增殖;凋亡;

    参考文献:
    [1]无症状高尿酸血症患者综合干预的疗效观察[J]. 邓卫,谢辉,张海红,谭晓军. 实用医学杂志. 2017(03)
    [2]内质网应激参与尿酸诱导的肾小管上皮细胞表型转化[J]. 吴璞,赵菲,牛丹,王新阳,郝亚宁. 中华肾脏病杂志. 2016 (12)
    [1] Crosstalk of autophagy and apoptosis:involvement of the dual role of autophagy under ER stress. Song S,Tan J,Miao Y,Li M,Zhang Q. Journal of Cellular Physiology . 2017
    [2] Global kidney health 2017 and beyond:a roadmap for closing gaps in care,research,and policy. Levin A,Tonelli M,Bonventre J,et al. The Lancet . 2017
    [3] Management of Chronic Kidney Disease:The Relationship Between Serum Uric Acid and Development of Nephropathy. Mende C. Advances in Therapy . 2015
    [4] Hyperuricemia is a biomarker of early mortality in patients with chronic obstructive pulmonary disease. Zhang X,Liu L,Liang R,et al. Int J Chron Obstruct Pulmon Dis . 2015
    [5] Associations Between Hyperuricemia and Chronic Kidney Disease:A Review. Sircar D,Chatterjee S,Waikhom R,et al. Nephrourol Mon . 2015
    [6] Endoplasmic reticulum stress,the unfolded protein response and autophagy in kidney diseases. Cybulsky AV. Nat Rev Nephrol . 2017
    [7] Japanese encephalitis virus induces apoptosis by the IRE1/JNK pathway of er stress response in BHK-21 cells. Huang M,Xu A,Wu X,et al. Archives of Virology . 2016
    [8] Chronic Kidney Disease. Webster AC,Nagler EV,Morton RL,et al. The Lancet . 2017
    [9] The physiology of uric acid and the impact of end-stage kidney disease and dialysis. MUREA M,TUCKER B M. Seminars in Dialysis . 2018
    [10] Treatment of asymptomatic hyperuricemia in chronic kidney disease:A new target in an old enemy-A review. RAMIREZ M,BARGMAN J M. J Adv Res . 2017

    肾小管上皮细胞尿酸增殖凋亡

  • 亚低温减轻肾上腺素对心肺复苏中心肌损伤及改善生存的影响

    作者:陶冉;甘伟妮;张洁;宋凤卿;左艳芳;陈蒙华; 期刊:《实用医学杂志》 2018年24期

    国家自然科学基金项目(编号:81460289,81201447); ;广西自然科学基金项目(编号:2013GXNSFAA019189); ;目的探讨亚低温治疗对肾上腺素在大鼠心肺复苏中心肌损伤及生存的影响。方法 32只雄性SD大鼠,建立心脏骤停/心肺复苏(CA/CPR)模型,随机分成4组(n=8):常温对照组(N)、常温肾上腺素组(N+E)、低温对照组(H)、低温肾上腺素组(H+E)。观察自主循环恢复情况、神经功能评分及存活情况。20只大鼠经造模后,随机分为N+E组和H+E组,在基础状态、复苏后0.5、1、2、4 h检测2组大鼠的血清心型脂肪酸结合蛋白,并在4 h处死大鼠取材观察心肌病理。结果 H+E组大鼠自主循环恢复率及72 h存活率明显高于N+E组及H组(P <0.05)。H+E组神经功能缺陷评分明显低于N+E组及H组(P <0.05)。H+E组大鼠脂肪酸结合蛋白明显低于N+E组(P <0.05),且复苏后4 h心肌病理损伤较N+E组明显减轻。结论肾上腺素和亚低温均能提高心脏骤停大鼠自主循环恢复成功率,但给予亚低温干预后,可明显改善肾上腺素复苏后早期心肌损伤,同时改善神经功能,延长存活时间。
    关键词:心脏骤停;心肺复苏;亚低温;肾上腺素;脂肪酸结合蛋白;
    基金:国家自然科学基金项目(编号:81460289,81201447); ;广西自然科学基金项目(编号:2013GXNSFAA019189); ;

    参考文献:
    [1]慢性心力衰竭合并心房颤动患者心型脂肪酸结合蛋白、超敏C反应蛋白及同型半胱氨酸水平的变化[J]. 林育辉,戴文军,何晓青. 实用医学杂志. 2018(08)
    [2]心型脂肪酸结合蛋白和N-末端脑钠肽前体在脓毒症心肌损伤中的变化及意义[J]. 齐洪娜,张建军,何佳起,王维展,李雅琴. 实用医学杂志. 2016(17)
    [3]亚低温联合μ-阿片受体激动剂对家兔心肺复苏后心功能的保护作用[J]. 张立平,李培杰,曹雯,张正义,罗延年. 西安交通大学学报(医学版). 2015(05)
    [4]亚低温对恢复自主循环后患者预后和脑功能的影响[J]. 保学明,卢建华,余涛,林俊敏. 实用医学杂志. 2015(03)
    [1]Ventricular fibrillation induced by transoesophageal cardiac pacing: A new model of cardiac arrest in rats[J] . Meng-Hua Chen,Tang-Wei Liu,Lu Xie,Feng-Qing Song,Tao He,Zhi-Yu Zeng,Shu-Rong Mo. Resuscitation . 2007 (3)
    [1] Activation of mitochondrial STAT-3and reduced mitochondria damage during hypothermia treatment for post-cardiac arrest myocardial dysfunction. Huang CH,Tsai MS,Chiang CY,et al. Basic Research In Cardiology . 2015
    [2] Lower-dose epinephrine administration and out-of-hospital cardiac arrest outcomes. Fisk CA,Olsufka M,Yin L,et al. Resuscitation . 2018
    [3] Heart-type fatty acid binding protein (H-FABP)as a biomarker for acute myocardial injury and long-term post-ischemic prognosis. Ye XD,He Y,Wang S,Wong GT,Irwin MG,Xia Z. Acta Pharmacological Sinica . 2018
    [4] Role of Cardiac Myocytes Heart Fatty Acid Binding Protein Depletion (H-FABP)in Early Myocardial Infarction in Human Heart (Autopsy Study). Shabaiek A,Ismael NEH,Elsheikh S,et al. Open access Maced J med Sci . 2016
    [5] A randomized trial of epinephrine in out-of-hospital cardiac arrest. PERKINS G D,JI C,DEAKIN C D,et al. The New England Journal of Medicine . 2018
    [6] Myocardial dysfunction and shock after cardiac arrest. JENTZER J C,CHONDE M D,DEZFULIAN C. Biomed Res Int . 2015
    [7] Epinephrine in cardiac arrest-insights from observational studies. ANDERSEN L W,GRANFELDT A. Resuscitation . 2018
    [8] Toxicological assessment of epinephrine and norepinephrine by analog approach. AYDIN A,TUGCU G. Food and Chemical Toxicology . 2018

    心脏骤停心肺复苏亚低温肾上腺素脂肪酸结合蛋白

  • 肾衰方及其拆方对肾间质纤维化Wnt4、β-catenin蛋白表达的影响

    作者:沈金峰;罗富里;黄伟;晏子友; 期刊:《实用医学杂志》 2018年24期

    国家自然科学基金(编号:81660769); ;江西省自然科学基金(编号:20161BAB205274); ;江西中医药大学硕士研究生创新专项资金(编号:JZYC18S24); ;目的观察中药肾衰方及其拆方对大鼠肾间质纤维化肾组织Wnt4、β-catenin蛋白表达的影响。方法将72只大鼠随机分为:假手术组、模型组、肾衰方组、祛邪方组、补虚方组、贝那普利组,采用单侧输尿管梗阻动物模型,术后7、14 d分别观察检测24 h尿蛋白定量、血清肌酐、尿素氮水平以及梗阻侧大鼠肾组织Wnt4、β-catenin含量的表达。结果 7、14 d时模型组大鼠24 h尿蛋白定量、血尿素氮、血清肌酐、Wnt4、β-catenin水平均高于假手术组(P <0.05),与模型组比较,经肾衰方、祛邪方、补虚方和贝那普利治疗后24 h尿蛋白定量、血尿素氮、血清肌酐、Wnt4、β-catenin水平明显降低(P <0.05),第14 d肾衰方优于其拆方(祛邪方、补虚方)及贝那普利(P <0.05)。结论肾衰方及其拆方可能是通过抑制Wnt4、β-catenin蛋白的表达,减轻肾间质的纤维化,改善肾功能,从而延缓慢性肾脏病进展,且肾间质纤维化中医为本虚标实之证。
    关键词:肾衰方;Wnt4;β-catenin;肾间质纤维化;
    基金:国家自然科学基金(编号:81660769); ;江西省自然科学基金(编号:20161BAB205274); ;江西中医药大学硕士研究生创新专项资金(编号:JZYC18S24); ;

    参考文献:
    [1]Wnt/β-catenin信号通路在肾缺血再灌注大鼠中的表达及ICG-001阻断对慢性肾间质纤维化的作用[J]. 黄健,刘畅,陈松,闵亚丽. 实用医学杂志. 2018(07)
    [2]浅谈Wnt/β-catenin与肾间质纤维化的关系[J]. 黄伟,沈金峰,谢娟,罗富里,晏子友. 江西医药. 2018(01)
    [3]Wnt信号通路与人类疾病相关性的研究进展[J]. 张遥,任秀智,韩金祥,鲁艳芹. 中国生物制品学杂志. 2018(01)
    [4]β连环蛋白翻译后修饰与肾间质纤维化[J]. 肖争,李瑛. 肾脏病与透析肾移植杂志. 2016(03)
    [5]弥漫型肾间质纤维化小鼠模型的快速制备及实验研究[J]. 李文歌,陈香美,师锁柱,郭瑞敏. 军医进修学院学报. 1996(03)
    [1]Experimental inhibition of porcupine mediated Wnt O-acylation attenuates kidney fibrosis[J] . Babita Madan,Mehul Patel,Jiandong Zhang,Ralph Bunte,Nathan P. Rudemiller,Robert Griffiths,David M. Virshup,Steven D. Crowley. Kidney International . 2016
    [2]LRP6 dimerization through its LDLR domain is required for robust canonical Wnt pathway activation[J] . Jinxiao Chen,Hongwei Yan,Dan-ni Ren,Yan Yin,Zhi Li,Qingqing He,Da Wo,Margaret Su-chun Ho,Yihan Chen,Zhongmin Liu,Jianhua Yang,Shangfeng Liu,Weidong Zhu. Cellular Signalling . 2014 (5)
    [3]Unilateral ureteral obstruction: beyond obstruction[J] . Alvaro C. Ucero,Alberto Benito-Martin,Maria C. Izquierdo,Maria D. Sanchez-Ni?o,Ana B. Sanz,Adrian M. Ramos,Sergio Berzal,Marta Ruiz-Ortega,Jesus Egido,Alberto Ortiz. International Urology and Nephrology . 2014 (4)
    [4]Wnt-signaling pathways in progressive renal fibrosis[J] . Peter J Nelson,Christine von Toerne,Hermann-Josef Grö,ne. Expert Opinion on Therapeutic Targets . 2011 (9)
    [1] Pathogenesis of chronic renal failure in the primary glomerulopathies, renal vasculopathies, and chronic interstitial nephritides. Bohle A,Muller G A,Wehrmann M,et al. Kidney International . 1996
    [2] TIMP-1 deficiency does not attenuate interstitial fibrosis in obstructive nephropathy. Kim H,Oda T,Lopez-Guisa J,et al. Journal of the American Society of Nephrology . 2001
    [3] Role of the Wnt/β-catenin signaling pathway in inducing apoptosis and renal fibrosis in 5/6-nephrectomized rats. Lin X,Zha Y,Zeng XZ,Dong R,Wang QH,Wang DT. Mol Med Rep . 2017
    [4] Sustained activation of Wnt/β-catenin signaling drives AKI to CKD progression. Xiao L,Zhou D,Tan R,et al. Journal of the American Society of Nephrology . 2016

    肾衰方Wnt4肾间质纤维化

  • 地衣芽孢杆菌对非酒精性脂肪肝病的干预作用及对肠黏膜通透性的影响

    作者:宋献美;吴晓东;石科;许波; 期刊:《实用医学杂志》 2018年24期

    河南省医学科技攻关计划(编号:201503234); ;目的探讨地衣芽孢杆菌对非酒精性脂肪性肝病(NAFLD)的干预作用和对肠黏膜通透性的影响。方法 Wistar大鼠随机分为正常(Con)组、模型(Mod)组、地衣芽孢杆菌低(BLL)、高(BLH)剂量组。大鼠用高脂饲料喂养复制NAFLD模型,第8周开始灌胃给药,末次给药后测定血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、总胆固醇(TC)、三酰甘油(TG)、二胺氧化酶(DAO)、内毒素(ETX)及肝匀浆中肿瘤坏死因子-α(TNF-α)的水平,免疫组化观察小肠中Occludin和ZO-1的表达。结果与Con组比较,Mod组血清中各生化指标水平明显升高,肝组织中TNF-α含量增加,小肠中Occludin和ZO-1蛋白表达明显降低(P <0.05);与Mod组相比,BLL、BLH组大鼠血清中ALT、AST、TC、TG、DAO及ETX含量明显降低,肝中TNF-α含量减少,肝组织病理学明显改善,小肠中Occludin和ZO-1的表达增加(P <0.05)。结论地衣芽孢杆菌可缓解NAFLD大鼠肝脏脂肪变性和炎症,其机制可能与增加小肠紧密连接蛋白的表达有关。
    关键词:地衣芽孢杆菌;非酒精性脂肪肝病;肠黏膜通透性;紧密连接;
    基金:河南省医学科技攻关计划(编号:201503234); ;

    参考文献:
    [1]姜黄素对非酒精性脂肪肝大鼠肠黏膜肌球蛋白轻链激酶的影响[J]. 侯洪涛,裘艳梅,张建,胡义亭,白云,王存凯,郑吉敏. 实用医学杂志. 2017(20)
    [2]Nonalcoholic fatty liver disease: Evolving paradigms[J]. Amedeo Lonardo,Fabio Nascimbeni,Mauro Maurantonio,Alessandra Marrazzo,Luca Rinaldi,Luigi Elio Adinolfi. World Journal of Gastroenterology. 2017(36)
    [3]Pediatric non-alcoholic fatty liver disease: Recent solutions, unresolved issues, and future research directions[J]. Maria Grazia Clemente,Claudia Mandato,Marco Poeta,Pietro Vajro. World Journal of Gastroenterology. 2016(36)
    [1] Overexpression of miR-429 im pairs intestinal barrier function in diabetic m ice by dow n-regulating occludin expression. YU T,LU X J,LI J Y,et al. Cell and Tissue Research . 2016
    [2] Supplementation with probiotics modifies gut flora and attenuates liver fat accumulation in rat nonalcoholic fatty liver disease model. Ren-ying Xu,Yan-ping Wan,Qi-yu Fang. JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION . 2012
    [3] The Role of Intestinal Bacteria Overgrowth in Obesity-Related Nonalcoholic Fatty Liver Disease. Ferolla SM,Armiliato GN,Couto CA,et al. Nutrients . 2014
    [4] Suppressed hepatic bile acid signalling despite elevated production of primary and secondary bile acids in NAFLD. JIAO N,BAKER SS,CHAPA-RODRIGUEZ A,et al. Gut . 2018
    [5] Current and upcoming pharmacotherapy for non-alcoholic fatty liver disease. Rotman Y,Sanyal AJ. Gut . 2017
    [6] Effects of compound Ginkgo biloba on intestinal permeability in rats with alcohol-induced liver injury. Li H,Qiu P,Wang J, et al. Food Funct . 2015
    [7] Pyrin inflammasome regulates tight junction integrity to restrict colitis and tumorigenesis. Sharma D,Malik A,Guy CS,et al. Gastroenterology . 2018
    [8] Burden of liver disease in Europe:Epidemiology and analysis of risk factors to identify prevention policies. Pimpin L,Cortez-Pinto H,Negro F, et al. Journal of Hepatology . 2018
    [9] Nonalcoholic Fatty Liver Disease,the Gut Microbiome,and Diet. Mokhtari,Z,D.L.Gibson,A.Hekmatdoost. Adv Nutr . 2017
    [10] The protective effects of Bacillus licheniformis preparation on gastrointestinal disorders and inflammation induced by radiotherapy in pediatric with central nervous system tumor. DU S X,JIA Y R,REN S Q,et al. Adv Med Sci . 2017

    地衣芽孢杆菌非酒精性脂肪肝病肠黏膜通透性紧密连接

服务与支付

加载页面耗时0.054秒